Composite

Part:BBa_K2443001

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-04)


ArgRS optimized for expression in E. coli

Arginine tRNA synthetase responsible for attaching asparagine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of ArgRs is 66 kDa, which corresponds with the band size shown in lane 5 in the overexpression gel below. Protein was overexpressed from BL21DE3 gold from pSB1C3 according to the protocols and notebook listed on the iGEM 2017 Lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf)

"BBa_K2443001-Oct_17_gel1.png"


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1805
    Illegal SapI.rc site found at 1523


[edit]
Categories
//collections/cell-free_tx-tl
Parameters
None